Uptake of LnPs by HeLa cells was analyzed by confocal and epifluorescence microscopies and by flow cytometry in the visible/NIR. Absorption/excitation/emission spectra, luminescence lifetimes, quantum yields and photostabilities were measured. (Ln 8-)G3P-(aza-BODIPY) n (n=16,32) were synthesized by coupling of aza-BODIPY with generation-3 polyamidoamine (G3P) dendrimer and interaction with Yb 3+ or Nd 3+ nitrates. The L1Nd 3+ complex was obtained by interaction between the ligand L1 and Nd 3+ nitrate (n=4,8) nanoparticles by encapsulation of 1,4- or 1,8- dihydroxyanthraquinones and Yb 3+ triflates in polystyrene beads and their functionalization with polyethylene glycol. Yb 3+ was synthesized by reaction of pyrazinehydroxamic acid with Zn 2+ and Yb 3+ triflates. 1 Herein, we present several families of LnPs. Low absorbance of free Ln 3+ requires the use of appropriate chromophores for sensitization of their luminescence. sharp emission bands not affected by the microenvironment and high resistance toward photobleaching. New generation of optical imaging agents, lanthanide(III) (Ln 3+)-based probes (LnPs), possess unique optical properties e.g. In particular, imaging in the near-infrared (NIR) region attracts significant attention due to reduced autofluorescence and light scattering. Fluorescence optical imaging is a highly sensitive technique.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |